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Registros recuperados: 59
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Pilot scale production of sea bass (Dicentrarchus labrax) fry ArchiMer
Bedier, Edouard.
Determination of rearing data enabled production of sea bass fry for three years in Station DEVA-SUD, CNEXO, France. The larval breeding process is described. The food consists of the ROlÎfer Brachionus plicatilis, then nauplii and 1 mm long metanauplii of the Brachiopod Artemia salina. Weaning on dry pellets oceurs when larvae are 35 days old when an optimum temperature curve is respected. Production of season 1977-78 was 380 000 two month old weaned fry.
Tipo: Text
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00342/45288/44752.pdf
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Ressources génétiques chez l'huître creuse Crassostrea gigas : variabilité, différenciation et adaptation aux conditions de production en Charente Maritime ArchiMer
Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie.
es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)...
Tipo: Text Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf
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Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris ArchiMer
Bierne, Nicolas; Bezuart, Ivan; Vonau, Vincent; Bonhomme, François; Bedier, Edouard.
Correlation between DNA microsatellite heterozygosity and growth rate was investigated in two hatchery-propagated stocks of the shrimp Penaeus stylirostris, which had been genetically isolated from wild founders for 17 generations, and from each other for five more generations. presumed demographic history of these populations suggested that they were maintained at small effective population sizes. and this was confirmed by significant changes in allelic frequencies between the two stocks. Despite a small sample size (n = 60) and a small number of loci screened, a significant positive correlation was detected between microsatellite tri-locus heterozygosity and growth rate in one of the two stocks (r = 0.3, P = 0.02). In the other stock (n = 48), the...
Tipo: Text Palavras-chave: Penaeus stylirostris; Heterozygosity; Marker associated heterosis; Inbreeding depression; Microsatellites.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-753.pdf
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Performance of Penaeus stylirostris After Six Generations of Selection for Growth ArchiMer
Goyard, Emmanuel; Penet, Laurent; Chim, Liet; Cuzon, Gerard; Bedier, Edouard; Bureau, D..
Tipo: Text
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00174/28479/26819.pdf
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Etude comparative en cytométrie en flux des fonctions estérases et du métabolisme oxydatif d'hémocytes d'huîtres saines, parasitées, sensibles et résistantes au protozoaire Bonamia ostrea ArchiMer
Cochennec, Nathalie; Garcia, Sandrine; Bedier, Edouard.
In order to fight Bonamiosis, the applicable means are very limited and mostly based on a quick and precocious diagnostic aimed at avoiding the propagation of the disease and a rise in the "resistance" through genetic selection. Various works are conducted in order to understand the oysters' defence mechanisms against this parasite, and then to develop new prophylaxis and fighting methods. In our laboratory, we focus on cellular compartments, the circulating haemocytes, in which B. ostreae multiplies, and their interaction with the parasite.
Tipo: Text Palavras-chave: Protozoaire; Huître; Hémocytes; Cytométrie en flux; Bonamia ostreae; Biologie cellulaire.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3275.pdf
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L'amélioration des performances de l'huître plate européenne Ostrea edulis par la sélection génétique ArchiMer
Bedier, Edouard; Baud, Jean-pierre; Cochennec, Nathalie; Cornette, Florence; Gerard, Andre; Goyard, Emmanuel; Joly, Jean-pierre; Kopp, Joel; Langlade, Aime; Le Coguic, Yvette; Mazurie, Joseph; Nourry, Max.
The European flat oyster Ostrea edulis has been considered as the traditional French oyster for a very long time and has now nearly disappeared from our farms, as a consequence of the two successive epizootic of the Marteilia refringens parasite and Bonamia ostreae. The actual quasi monoculture of Crassostrea gigas has made necessary the search for a diversification in oyster culture, including the search for a new start of the indigenous flat oyster culture. The Ifremer has been conducting parallel works since 1985 in order to better understand the biology of the parasite as well as to try and select different lines of flat oyster resistant to Bonamia ostreae.
Tipo: Text Palavras-chave: Bonamia ostrea; Huître plate; Sélection Génétique; Ostrea edulis.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3274.pdf
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Genetical basis of the plasticity of resource allocation in the Pacific oyster (Crassostrea gigas) ArchiMer
Ernande, Bruno; Haure, Joel; Degremont, Lionel; Bedier, Edouard; Boudry, Pierre.
Phenotypic and genetic correlations between fitness-related life-history traits - such as survival, growth and reproductive allocation - have to be considered to better understand selective processes, both in the wild and in breeding programs. Quantitative genetic experiments, based on nested half-sib mating designs, were perfomed in the Pacific oyster C. gigas (1) to estimate genetic variance and the response to selection and (2) to document phenotypic and genetic trade-offs between life history traits. Physiological trade-offs, i.e. plastcity of resource allocation, were shown to vary among genotypes. Genetic polymorphism was primarily observed for the the plasticity of reproductive effort. In addition, the degree of plasticity in reproductive effort...
Tipo: Text Palavras-chave: Genetic polymorphism; Genetic; Plasticity; Genetical basis; Crassostrea gigas; Pacific oysters.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3443.pdf
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Genetic basis of summer mortality in juvenile cupped oysters ArchiMer
Degremont, Lionel; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Soletchnik, Patrick; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois.
The French project "Morest", coordinated by IFREMER, aims to understand the causes of the summer mortalities in Crassostrea gigas. In 2001, three sets of families were bred following a nested half-sib mating design. 17 half-sib families (HSF) were obtained in this first generation (G1) and reared in 3 sites. Significant differences in survival were observed among HSF, and some HSF showed high levels of mortality in all sites, clearly indicating a genetic basis for survival. In 2002, a second generation (G2), including divergent selection and inbred lines, was constituted. Monitoring of survival and growth of G2s were the same as in 2001. Significant differences in survival were found between the offspring of the "high" and "low" selected groups and between...
Tipo: Text Palavras-chave: Summer; Oyster culture; Juveniles mortality; Genetics; Commercial species; Breeding.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3346.pdf
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Pratiques culturales et mortalités d'huîtres creuses Crassostrea gigas en baie de Quiberon, en 2001-2002. Bilan des enquêtes, plongées et prélèvements sur 18 semis en 2001-2002 ArchiMer
Mazurie, Joseph; Langlade, Aime; Fleury, Pierre-gildas; Bouget, Jean-francois; Claude, Serge; Foucart, Marie; Bedier, Edouard.
This study is the second part of a former report rapport Ifremer DRV/RST/RA/LCB/2002-08 dealing with rearing methods, growth and mortality performances, and problems encountered by the oyster farmers from Quiberon Bay (2800 ha of leases) : the first report relied on a survey achieved in july 200l, about 18 oyster farmers, and 2 diving campaigns (july and october 2001) on 2 years old oyster batches from the same farmers. Il is now completed by a last campaign of diving in october 2002, on 7 parcels out of the 18, still bearing the initial oysters. The analysed enterprises vary in particular by the location and depth of their concession inside the bay, but also by their rearing scheme : a majority favours seedings with 18 month old oysters or more, according...
Tipo: Text Palavras-chave: Huître creuse; Crassastrea gigas; Baie de Quiberon; Croissance; Mortalité; Pratiques culturales; Rentabilité économique; Cupped oyster; Crassostrea gigas; Mortality; Rearing practices; Profitability; Bay of Quiberon; .growlh.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00102/21322/18944.pdf
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Characterization of summer mortalities of C. gigas oyster in France in relation to environmental parameters ArchiMer
Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Costil, K. S.; Dubois, Brice; Degremont, Lionel; Bouget, Jean-francois; Martin, Jean-louis; Enriquez-diaz, M.; Faury, Nicole; Le Moine, Olivier; Renault, Tristan; Gagnaire, Beatrice; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois.
Field characterization of summer mortality was performed in France in the frame of the Morest project. Natural and hatchery spat were compared between three oyster production areas in France. Regardless of the natural or hatchery origin, oysters died during the reproduction period after temperature reaches 19 degree C. Thus, in southern areas, temperature accelerated gametogenesis of small spat (10mm) as well as adults, and mortality appeared for the two age classes. In contrast, sexual maturation proceeded more slowly in northern where spat mortality was lower compared to 18 months old oysters. However, critical gametogenesis and temperature were not sufficient to induce mortalities, as observed in examples with stable environment. Alternatively sediment...
Tipo: Text Palavras-chave: Seasonal variation; Oyster culture; Mortality causes; Hatcheries; Gametogenesis; Environmental factors; Crassostrea gigas; Oysters.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3348.pdf
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Mortalités estivales et croissance du naissain de l'huître creuse Crassostrea gigas : étude de familles sélectionnées ArchiMer
Degremont, Lionel; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Soletchnik, Patrick; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois.
In the scope of the Morest program, 3 series of bi-parental families of cupped oyster consisting of full and half-sib have been created in 2001, and then studied on a regular basis on several sites over the summer period. In October, major survival differences had been demonstrated between the 3 families. The average survival rate for the half-sib families monitored on the three sites varies from 17 to 97 %. Some of them showed high mortality for the three sites, indicating a strong genetic basis (h2 = 0.81 - 0.29) to the variations observed regarding survival. To a lesser extent, growth differences between the studied half-sib families indicate a weaker heritability for this character. ....
Tipo: Text Palavras-chave: Physiologie; Génétique; Naissain; Sélection; Croissance; Survie; Huîtres; Crassostrea gigas.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3490.pdf
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Summer mortality in juvenile cupped oysters : response to selection in the field and in laboratory ArchiMer
Degremont, Lionel; Bedier, Edouard; Soletchnik, Patrick; Ropert, Michel; Samain, Jean-francois; Boudry, Pierre.
The French project "Morest", coordinated by IFREMER, aims to a better understanding of the causes of summer mortalities in juvenile oysters by a multidisciplinary approach. Within this program, a first generation (G1) was bred in 2001 to assess to what extent genetic variability exists for this trait in French populations. 17 half-sib families (HSF) including 44 full-sib families (FSF) were obtained in the G1, and each FSF was reared in three sites along the French coast during the summer period. Significant differences in survival were observed among HSF, clearly indicating a genetic basis for survival. Estimation of heritability for survival was very high: 0.81  0.29. In 2002, 3 "good" HSF and 3 "bad" HSF were selected on their survival...
Tipo: Text Palavras-chave: Selection; Genetic; Mortalities; Crassostrea gigas; Oyster.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3439.pdf
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Genetic and reproduction strategy in Crassostrea gigas mortality ArchiMer
Moal, Jeanne; Bedier, Edouard; Fleury, Pierre-gildas; Langlade, Aime; Le Coguic, Yvette; Degremont, Lionel; Boudry, Pierre; Le Coz, Jean-rene; Pouvreau, Stephane; Enriquez-diaz, M.; Lambert, Christophe; Quere, Christele; Soudant, Philippe; Samain, Jean-francois.
Bi-parental families were produced in hatchery and tested in the field in 2001. Two sets of 5 families were constituted, selected on their high (R) and low (S) survival. These two sets were reared in Brittany from March to November 2002. Samplings were performed twice a month to obtain data on biometry, survival, reproductive cycle, biochemical composition, adenylate energy charge, hemolymph parameters (ions and defense system) and muscle strength. "R" and "S" oysters exhibited different reproductive effort and spawning strategy. "R" oysters allocated less energy in gonad than "S" ones and presented a complete spawning at the end of August contrary to the "S" which spawns partially. Mortality started in July when the seawater temperature reached 19 degree...
Tipo: Text Palavras-chave: Summer; Reproduction; Oyster culture; Mortality; Genetic diversity; Commercial species; Breeding.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3345.pdf
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Selective breeding to improve resistance against summer mortality in the Pacific oyster Crassostrea gigas : results after 3 generations ArchiMer
Boudry, Pierre; Degremont, Lionel; Bedier, Edouard; Samain, Jean-francois.
Summer mortality of adults and juveniles has been reported in the Pacific oyster, Crassostrea gigas, for many years and in several countries. The French multidisciplinary program "Morest" aims to investigate the causes of the summer mortality in Crassostrea gigas. Within this program, we designed multi-site field experiments to assess to what extent genetic variability exists for summer mortality in French populations of C. gigas and to determine whether selective breeding could improve survival.
Tipo: Text Palavras-chave: Growth; Resistance; Selective breeding; Genetic; Crassostrea gigas; Pacific oyster; Summer mortality.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3448.pdf
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Le risque thermique : caractérisation des environnements thermiques des 3 sites atliers de MOREST en lien avec les mortalités observées sur le terrain ArchiMer
Ropert, Michel; Rauflet, Fabienne; Soletchnik, Patrick; Geairon, Philippe; Bedier, Edouard; Bouget, Jean-francois.
Temperature affects undoubtedly the 3 working sites where, systematically, the observation of the first abnormal mortalities coincides with a water temperature of around 19°C. The nature of the processes implied could have a "cumulative" effect, affecting the physiology (this phenomenon has been observed with other marine species) or a "threshold" effect, acting only as a trigger. The question has not been answered yet. The kinetics of the thermic evolution around the mortality period has to be studied in depth in order to understand if this temperature acts more as an "associated factor", during a chronic stress period, or during an acute stress, as suggested by the "on-bottom/off bottom" mortality model of the Marennes Oléron Bay [12].
Tipo: Text Palavras-chave: MOREST; Mortalité; Stress; Température; Risque thermique; Physiologie; Crassostrea gigas; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3408.pdf
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Apport d'un programme de génétique à une filière de production aquacole : l'exemple de l'ostréiculture ArchiMer
Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre.
Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between...
Tipo: Text Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf
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Le risque sédimentaire ArchiMer
Bedier, Edouard; Fleury, Pierre-gildas; Langlade, Aime; Haure, Joel; Le Roux, A.; Ropert, Michel; Rauflet, Fabienne; Soletchnik, Patrick.
The "sedimentary risk" has been identified since the mid-nineties in the Marennes Oléron Bay. The proximity of the sediment (from 0 to 15cms) increases the mortality rate. Depending on the year, mortality can be 10 to 20 % higher than in "high" conditions (50 to 70cms). These zootechnic results, gathered since 1996 on the working site of Ronces – Perquis, lead to the research of a pathogenic agent or a toxic substance released by the sediment. Studies conducted between 2002 and 2003 [12, 18] did not allow the identification of any pathogenic agents and ruled out the infectious risk. In 2004, the chemical risk (sulphides, ammoniacal nitrogen) was studied on 4 sites (3 working sites + Quiberon). In 2004, the "on-bottom/off bottom" mortality model of the...
Tipo: Text Palavras-chave: MOREST; Hydrologie; Zootechnie; Sédimentologie; Mortalités; Crassostrea gigas; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3411.pdf
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Bilan 2003 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins ArchiMer
Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Kopp, Joel; Mouillard, Gilbert; Gerla, Daniel; Le Saux, Jean-claude; Raguenes, Pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Fillon, Alain.
The REPAMO network (network ofmollusc pathology), created in 1986, ensures the survey of shellfish health status along French coasts according? to European Directives 91/67/EEC and 95/70/EEC. The aims of the network are 1 - to prevent the introduction and spread of exotic pathogens, 2-to study the means to decrease the impact of pathogens already present and to survey their evolution. In 2003, the REPAMO network focused on the survey oflisted diseases (bonamiosis and marteiliosis) in two areas under agreement process (Banc de Granville and zone X) and on the study of abnormal morlalities. Number of reported abnormal morlalities has increased in 2003. This rise can be partly explained by the recent network restructuring, but also by the summer heatwave and...
Tipo: Text Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00086/19699/17340.pdf
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Effects of age and environment on the summer mortality in cupped oyster Crassostrea gigas during the first two years ArchiMer
Degremont, Lionel; Boudry, Pierre; Soletchnik, Patrick; Bedier, Edouard; Ropert, Michel; Samain, Jean-francois.
Three successive generations were produced between 2001 and 2003 to assess to what extent genetic variability exists for survival in juvenile oysters Crassostrea gigas. For each generation, two groups were selected for their high ("R" for resistant) and low ("S" for susceptible) survival. Significant differences in mortality were observed during the first year in Riviere d'Auray (RA) (Brittany-France). However, low and similar mortality were observed for both groups, with no significant difference between groups in the first and second generation in this site, where the first summer is the critical, sensitive period with a clear age effect. In Baie des Veys (BDV) (Normandie-France), where mortalities usually mainly affect adults, mortality between groups,...
Tipo: Text Palavras-chave: Crassostrea gigas; Spat; Spatial variations; Survival; Rearing; Mollusc culture; Marine molluscs; Environmental factors; Nursery grounds; Mortality causes; Biological age; Aquaculture techniques.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3385.pdf
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The identification of genes from the oyster Crassostrea gigas that are differentially expressed between progeny exhibiting opposed susceptibility to summer mortality ArchiMer
Huvet, Arnaud; Degremont, Lionel; Labreuche, Yannick; Daniel, Jean-yves; Dubrunfaut, Thomas; Boudry, Pierre; Haure, Joel; Bedier, Edouard; Ropert, Michel; Herpin, Amaury; Cunningham, Charles; Moal, Jeanne; Samain, Jean-francois.
Summer mortality associated with juveniles of the oyster Crassostrea gigas is the result of a complex interaction between the host, pathogens and environment. Divergent selection based on summer survival have been applied to produce families with resistant and susceptible progeny. Here, we describe the use of suppression subtractive hybridization to generate 150 clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these 150 clones was determined. Among the 25 unique sequences that matched with the products of known genes, those putatively implicated in energy generation (12%) and immune function (16%), systems suspected to be implicated in summer mortality, were extended and analyzed by real...
Tipo: Text Palavras-chave: Selection; Gene; Genetic; Summer mortality; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3447.pdf
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